Tests such as dissolution, content uniformity, assay, and related substances require sample filtration prior to HPLC / UHPLC analysis. Since quantitation of analytes is critical following these tests, filter validation studies should be used to evaluate analyte loss due to membrane filter adsorption. Different membrane filters can bind analyte to varying degrees depending on membrane and analyte type, as well as analyte concentration.
The objective of this video is to provide guidance on filter selection during method development and validation with a special emphasis on analyte binding to syringe filters.