The University of Guelph is located in the Southern part of the City of Guelph and is known as one of the world's top schools for it's veterinary medicine program. It's main campus contains the 165-hectare University of Guelph Arboretum and a bus terminal which serves both local and intercity bus service. It is found at the South-west corner of the campus near the intersection of Stone Rd & Gordon St.

Buses seen include:
Guelph Transit:
249 on Route 1
267 on Route 2
228 on Routes 2 & 15
273 & 292 on Route 5
234 & 286 on Route 6
234 on Route 7
228 & 267 on Route 15
203 & 281 on Route 17
211 & 246 on Route 18
220, 242, 244, 254, 272, 280, & 285 on Route 99
205 was Not in Service

GO Transit:
2546 & 8429 on Route 17
2526 on Route 29
8432 on Route 33
8556 was Not In Service

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