This is a copy of the talk given at the Crick Institute London on 28th June 2019 entitled: Optimized processing and analysis of conventional confocal microscopy generated scanning FCS data. It contains background explanation describing Fluorescence Correlation Spectroscopy (FCS) and scanning FCS. These are both techniques which can be used to measure the rate of diffusion of biological molecules which have been tagged with some kind of fluorescence fluorophore. During the presentation I introduce scanning FCS software called (FoCuS-scan) which an be found here: https://github.com/dwaithe/FCS_scanni...) and specifically the manual of this software which can be found here: https://github.com/dwaithe/FCS_scanni...
. Finally, the journal publication which describes much of this content is open-access and can be found here: https://www.sciencedirect.com/science...