This video demonstrates how to perform required, optional, and advanced alignments for a JEOL 2100 S/TEM with a LaB6 electron source. Required alignments include correcting the Z height, condenser lens aperture centering, and voltage axis centering. Optional alignments include intermediate lens centering (for electron diffraction), condenser lens astigmatism (for high resolution imaging), and objective lens astigmatism (for high resolution imaging). Advanced alignments include gun tilt, gun shift, tilt purity, and shift purity.