Step-by-step instructions for preparing ionically crosslinked alginate hydrogels. Includes loading alginate and calcium solutions into syringes, removing bubbles, mixing between syringes, depositing alginate between plates, and punching out gels with a biopsy punch.

For more information, visit the associated protocol: Tuning Viscoelasticity in Alginate Hydrogels for 3D Cell Culture Studies
https://currentprotocols.onlinelibrar...