Introduction
Technique by which tissues can be sliced into thin sections and then attached to a surface for microscopy

Microtomes. These are instruments used to cut uniformly thin sections of the tissues for further study.

Microtomes use sharp glass or steel blades/diamond knives to cut the tissues into thin sections of uniform thickness

The thickness of the sections is predetermined at a regular distance on the microtome

Types of Microtomy
Main types include
Rotary microtome
Ultramicrotome
Freezing microtome
Cryotome
Use depends on the material under study and the nature of the work
Simple studies involve rotary microtome while detailed studies may involve ultramicrotome, etc.
Similarly, cryomicrotome involves sectioning of the frozen tissues.
Sample preparation for Optical Microscopy
It consists of the following steps:
Fixation
Processing
Dehydration
Clearing
Embedding
Section cutting-microtomy
Staining
Deparaffinization

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