The typical process used in synthetically creating DNA is the phosphoramidite method. This method is replicated in the 3'- to 5'- direction which is opposite to that in nature. One amidite base is added per synthesis cycle. When the sequence is completed the DNA is separated from the solid support and the base protecting groups are removed.

The following video outlines this process and covers basic questions regarding the chemistry and post synthesis operations. For more information regarding this process or a custom developed process please contact us at biolytic.com.

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TIMESTAMPS:
00:00 - Introduction To Oligo Synthesis
00:15 - Oligo Synthesis Cycle
01:21 - Start Detritylation
01:56 - What are the differences between using DCA and TCA?
02:36 - Which acid (TCA or DCA) is faster for detritylation?
02:51 - Why is DCA not primarily used over TCA?
03:10 - Which chemical is easier to work with (TCA or DCA)?
03:44 - Step 001 - Activation and Coupling
04:20 - What is the reason for the activator solution?
04:37 - What is the difference between a universal solid support and a standard solid support?
04:56 - How long does it take for the chemical reactions to occur?
05:26 - Step 002 - Capping
06:33 - Step 003 - Oxidation
07:05 - Can backbone modifications be done as a replacement for the oxidation step?
07:26 - Wash Step
07:51 - Post Synthesis Overview
08:51 - Post Synthesis Walkthrough - Cleave + Elute - Deprotection - Oligo Quantification
09:32 - How long does the deprotection process take with an elevated temperature?
10:06 - Oligo Quantification
10:32 - Post Synthesis Complete