There are 2 main modes of transport of molecules across any biological membrane. These are passive and active transport. Passive transport, most commonly by diffusion, occurs along a high-to-low concentration gradient. No energy is necessary for this mode of transport. Examples will include the diffusion of gases across alveolar membranes and the diffusion of neurotransmitters such as acetylcholine across the synapse or neuromuscular junction. Osmosis is a form of passive transport when water molecules move from low solute concentration (high water concentration) to high solute or low water concentration across a membrane that is not permeable to the solute. There is a form of passive transport called facilitated diffusion. It occurs when molecules such as glucose or amino acids move from high concentration to low concentration facilitated by carrier proteins or pores in the membrane. Active transport requires energy for the process by transporting molecules against a concentration or electrochemical gradient. Active transport is an energy-driven process where membrane proteins transport molecules across cells, mainly classified as primary or secondary, based on how energy is coupled to fuel these mechanisms. The former constitutes the means by which a chemical reaction, e.g., ATP hydrolysis, powers the direct transport of molecules to establish specific concentration gradients, as seen with sodium/potassium-ATPase and hydrogen-ATPase pumps. The latter employs those established gradients to transport other molecules. These gradients support the roles of other membrane proteins and other workings of the cell and are crucial to maintaining cellular and bodily homeostasis. As such, the importance of active transport is apparent when considering the various defects throughout the body that can manifest in a wide variety of diseases, including cystic fibrosis and cholera, all because of an impairment in some aspect of active transport.