Multiple technology platforms are available to support high-quality antibody development in both research and drug discovery. Single B cell sorting has been widely used for antibody screening with advantages in allowing the isolation of native and functional antibodies within a higher chance. Sino Biological Inc. uses this platform to develop rabbit and mouse monoclonal antibodies by coupling antigen-specific single B cell sorting followed by antibody sequence PCR or B cell culture. We successfully obtained rabbit monoclonal antibodies targeting SARS-CoV-2 key antigen, spike protein, with blocking functions.